The Expanding Family of Natural Anion Channelrhodopsins Reveals Large Variations in Kinetics, Conductance, and Spectral Sensitivity
Identifieur interne : 000018 ( Main/Exploration ); précédent : 000017; suivant : 000019The Expanding Family of Natural Anion Channelrhodopsins Reveals Large Variations in Kinetics, Conductance, and Spectral Sensitivity
Auteurs : Elena G. Govorunova [États-Unis] ; Oleg A. Sineshchekov [États-Unis] ; Elsa M. Rodarte [États-Unis] ; Roger Janz [États-Unis] ; Olivier Morelle [Allemagne] ; Michael Melkonian [Allemagne] ; Gane K.-S. Wong [Canada, République populaire de Chine] ; John L. Spudich [États-Unis]Source :
- Scientific Reports [ 2045-2322 ] ; 2017.
Abstract
Natural anion channelrhodopsins (ACRs) discovered in the cryptophyte alga
Url:
DOI: 10.1038/srep43358
PubMed: 28256618
PubMed Central: 5335703
Affiliations:
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Spudich</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Center for Membrane Biology, Department of Biochemistry & Molecular Biology, The University of Texas Health Science Center at Houston, McGovern Medical School</institution>, Houston, Texas,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">28256618</idno><idno type="pmc">5335703</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5335703</idno><idno type="RBID">PMC:5335703</idno><idno type="doi">10.1038/srep43358</idno><date when="2017">2017</date><idno type="wicri:Area/Pmc/Corpus">000205</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000205</idno><idno type="wicri:Area/Pmc/Curation">000205</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000205</idno><idno type="wicri:Area/Pmc/Checkpoint">000003</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000003</idno><idno type="wicri:Area/Ncbi/Merge">002235</idno><idno type="wicri:Area/Ncbi/Curation">002235</idno><idno type="wicri:Area/Ncbi/Checkpoint">002235</idno><idno type="wicri:Area/Main/Merge">000018</idno><idno type="wicri:Area/Main/Curation">000018</idno><idno type="wicri:Area/Main/Exploration">000018</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The Expanding Family of Natural Anion Channelrhodopsins Reveals Large Variations in Kinetics, Conductance, and Spectral Sensitivity</title><author><name sortKey="Govorunova, Elena G" sort="Govorunova, Elena G" uniqKey="Govorunova E" first="Elena G." last="Govorunova">Elena G. Govorunova</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Center for Membrane Biology, Department of Biochemistry & Molecular Biology, The University of Texas Health Science Center at Houston, McGovern Medical School</institution>, Houston, Texas,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Sineshchekov, Oleg A" sort="Sineshchekov, Oleg A" uniqKey="Sineshchekov O" first="Oleg A." last="Sineshchekov">Oleg A. Sineshchekov</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Center for Membrane Biology, Department of Biochemistry & Molecular Biology, The University of Texas Health Science Center at Houston, McGovern Medical School</institution>, Houston, Texas,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Rodarte, Elsa M" sort="Rodarte, Elsa M" uniqKey="Rodarte E" first="Elsa M." last="Rodarte">Elsa M. Rodarte</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Department of Neurobiology & Anatomy, The University of Texas Health Science Center at Houston, McGovern Medical School</institution>, Houston, Texas,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Janz, Roger" sort="Janz, Roger" uniqKey="Janz R" first="Roger" last="Janz">Roger Janz</name><affiliation wicri:level="1"><nlm:aff id="a2"><institution>Department of Neurobiology & Anatomy, The University of Texas Health Science Center at Houston, McGovern Medical School</institution>, Houston, Texas,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Morelle, Olivier" sort="Morelle, Olivier" uniqKey="Morelle O" first="Olivier" last="Morelle">Olivier Morelle</name><affiliation wicri:level="1"><nlm:aff id="a3"><institution>Institute of Botany, Cologne Biocenter, University of Cologne</institution>, Cologne,<country>Germany</country></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Melkonian, Michael" sort="Melkonian, Michael" uniqKey="Melkonian M" first="Michael" last="Melkonian">Michael Melkonian</name><affiliation wicri:level="1"><nlm:aff id="a3"><institution>Institute of Botany, Cologne Biocenter, University of Cologne</institution>, Cologne,<country>Germany</country></nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Wong, Gane K S" sort="Wong, Gane K S" uniqKey="Wong G" first="Gane K.-S." last="Wong">Gane K.-S. Wong</name><affiliation wicri:level="1"><nlm:aff id="a4"><institution>Departments of Biological Sciences and of Medicine, University of Alberta, Edmonton</institution>, Alberta,<country>Canada</country></nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="a5"><institution>BGI-Shenzhen</institution>, Shenzhen,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Spudich, John L" sort="Spudich, John L" uniqKey="Spudich J" first="John L." last="Spudich">John L. Spudich</name><affiliation wicri:level="1"><nlm:aff id="a1"><institution>Center for Membrane Biology, Department of Biochemistry & Molecular Biology, The University of Texas Health Science Center at Houston, McGovern Medical School</institution>, Houston, Texas,<country>USA</country></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">Scientific Reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Natural anion channelrhodopsins (ACRs) discovered in the cryptophyte alga <italic>Guillardia theta</italic> generate large hyperpolarizing currents at membrane potentials above the Nernst equilibrium potential for Cl<sup>−</sup> and thus can be used as efficient inhibitory tools for optogenetics. We have identified and characterized new ACR homologs in different cryptophyte species, showing that all of them are anion-selective, and thus expanded this protein family to 20 functionally confirmed members. Sequence comparison of natural ACRs and engineered Cl<sup>−</sup>-conducting mutants of cation channelrhodopsins (CCRs) showed radical differences in their anion selectivity filters. In particular, the Glu90 residue in channelrhodopsin 2, which needed to be mutated to a neutral or alkaline residue to confer anion selectivity to CCRs, is nevertheless conserved in all of the ACRs identified. The new ACRs showed a large variation of the amplitude, kinetics, and spectral sensitivity of their photocurrents. A notable variant, designated “ZipACR”, is particularly promising for inhibitory optogenetics because of its combination of larger current amplitudes than those of previously reported ACRs and an unprecedentedly fast conductance cycle (current half-decay time 2–4 ms depending on voltage). ZipACR expressed in cultured mouse hippocampal neurons enabled precise photoinhibition of individual spikes in trains of up to 50 Hz frequency.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Deisseroth, K" uniqKey="Deisseroth K">K. Deisseroth</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, F" uniqKey="Zhang F">F. Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chow, B Y" uniqKey="Chow B">B. Y. Chow</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maurice, N" uniqKey="Maurice N">N. 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Sineshchekov</name><name sortKey="Spudich, John L" sort="Spudich, John L" uniqKey="Spudich J" first="John L." last="Spudich">John L. Spudich</name></country><country name="Allemagne"><noRegion><name sortKey="Morelle, Olivier" sort="Morelle, Olivier" uniqKey="Morelle O" first="Olivier" last="Morelle">Olivier Morelle</name></noRegion><name sortKey="Melkonian, Michael" sort="Melkonian, Michael" uniqKey="Melkonian M" first="Michael" last="Melkonian">Michael Melkonian</name></country><country name="Canada"><noRegion><name sortKey="Wong, Gane K S" sort="Wong, Gane K S" uniqKey="Wong G" first="Gane K.-S." last="Wong">Gane K.-S. Wong</name></noRegion></country><country name="République populaire de Chine"><noRegion><name sortKey="Wong, Gane K S" sort="Wong, Gane K S" uniqKey="Wong G" first="Gane K.-S." last="Wong">Gane K.-S. Wong</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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